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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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References: Glutathione and Parkinsons Disease: Glutathione metabolism in type 2 diabetes and its relationship with microvascular complications and glycemia Glutathione supplementation improves oxidative damage in experimental colitis Application of Glutathione as Anti-Oxidative and Anti-Aging Drugs Differentiating Sulfur Compounds Sulfa Drugs, Glucosamine Sulfate, Sulfur, and Sulfiting Agents by Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, Portland, Oregon

While the antioxidant properties of glutathione may offer protective benefits, it's essential to approach supplementation thoughtfully, especially when on medication

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