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They were kept in a 12-hour light-dark cycle (lights on at 8:00 am and lights off at 8:00 pm) with free access to sterilized food and water, grouped together throughout the experiment

NTBI can cross the plasma membrane and especially easily be absorbed by various cells, such as cardiomyocytes through calcium channels, causing cascade damage

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Recovery typically requires 12 weeks, after which metabolic management can proceed without gallbladder-specific constraints