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Identification of glucocorticoid-regulated genes that control cell proliferation during murine respiratory development

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46 In keeping with this, transgenic mice overexpressing GIP exhibit increased bone mass and enhanced osteoblast activity at 24 months of age, whilst GIPR KO leads to decreased BMD, cortical thickness, and overall bone strength

Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution