Finally, development was performed using a high-sensitivity enhanced chemiluminescence reagent (Amersham Pharmacia Biotech, UK), and the grayscale values were analyzed by ImageJ software
2.2 Patient 2 A 39-year-old female with PWS resulting from mosaic maternal uniparental disomy was evaluated at our Endocrinology Unit
C8 and C10 had no effect in physiological concentrations (2 mM), although at higher concentrations C10 had a stronger HDAC-inhibitory activity than C8
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